Determination of E Vitamins in the Flowers and Pollens of Fritillaria thunbergii Miq. by HPLC-FLD

【Author】

JIANG Xin-hang1, LIANG Jun-ling2, LI Jian-wei3, WU Shi-hua2* 1. Equipment & Technology Service Platform, College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310058, China; 2. Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Science, Hangzhou, Zhejiang 310058, China; 3. Ningbo Tianyun Agricultural Development Co., Ltd, Ningbo, Zhejiang 315192, China

【Abstract】

[Objective] To analyze and determine the chemical structures and contents of E vitamins in flowers and pollens of F. thunbergii Miq., so as to provide scientific basis for its further development. [Methods] High-performance liquid chromatography-florescence detection (HPLC-FLD) was first established to determine the compositions of E vitamins. The detection conditions were as follows: Agilent Zorbax RX-SIL column (4.6 mm×150 mm, 5 μm) and guard column (4.6 mm ×10 mm, 5 μm), mobile phase of hexane-0.2% isopropanol-0.8% acetic acid , column temperature of 25 ℃, flow rate of 1.0 ml/min, the maximum excitation and emission wavelengths were 292 and 328 nm.[Results]8 kinds of vitamins were determined simultaneously for the first time, which were in good linear relationship within the detection range, with R2>0.999 0 and recovery rate exceeded 98%. There were 7 kinds of E vitamins in the flower of F. thunbergii Miq., among which D-α-tocotrienols was the highest that reached 181.606 mg/kg, however the contents of E vitamins in the pollens of F. thunbergii Miq. were relatively lower. [Conclusion] There are multiple kinds of E vitamins in the flowers and pollens of F. thunbergii Miq., comparatively speaking, E vitamins in the flower of F. thunbergii Miq. were more than that in pollen, which deserves further research. Besides, HPLC-FLD is convenient and efficiency that can be used for determining the contents of E vitamins.

【Keywords】

Flowers of F. thunbergii Miq., Vitamin E, HPLC-FLD, China

References

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Total: 16 articles

  • [1] WANG Ruiying, TANG Jing, ZHANG Lijing ,WANG Qiang (Physics and Chemistry Detecting Center of Xinjiang University,Urumqi,Xinjiang 830046, China), Determination of Vitamin E Contents of a Variety of Plant Oils by High Performance Liquid Chromatography (HPLC), Journal of Xinjiang University (Natural Science Edition),
  • [2] SHAO Cheng-bin, TAN Dong-mei,LI Ren-bing, CAI Qiang (Environmental and Biological Engineering College ,Chongqing Technology and Business University,Chongqing,400033,China), Determination of vitamin E in Barleygreen by spectrophotography, Journal of Yuzhou University (Natural Sciences Edition),
  • [3] 1, 4XIAO Pei-Gen 2JIANG Yan 2LI Ping 3 LUO Yi-Bo 4LIU Yong 1 (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China) 2 (Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China) 3 (State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China) 4 (School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China), The botanical origin and pharmacophylogenetic treatment of Chinese materia medica Beimu, Acta Phytotaxonomica Sinica,
  • [4] Chen Wenjun, Zheng Weihong,Xiang Yinghua Pharmaceutical Institute of Zhejiang Chinese Medical University,Hangzhou(310053), Content Measurement of Bulb Total Alkaloid and Saponin in Zhejiang Fritillariae and Thuubergii, Journal of Zhejiang University of Traditional Chinese Medicine,

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