JIANG Xin-hang1, LIANG Jun-ling2, LI Jian-wei3, WU Shi-hua2* 1. Equipment & Technology Service Platform, College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310058, China; 2. Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Science, Hangzhou, Zhejiang 310058, China; 3. Ningbo Tianyun Agricultural Development Co., Ltd, Ningbo, Zhejiang 315192, China
[Objective] To analyze and determine the chemical structures and contents of E vitamins in flowers and pollens of F. thunbergii Miq., so as to provide scientific basis for its further development. [Methods] High-performance liquid chromatography-florescence detection (HPLC-FLD) was first established to determine the compositions of E vitamins. The detection conditions were as follows: Agilent Zorbax RX-SIL column (4.6 mm×150 mm, 5 μm) and guard column (4.6 mm ×10 mm, 5 μm), mobile phase of hexane-0.2% isopropanol-0.8% acetic acid , column temperature of 25 ℃, flow rate of 1.0 ml/min, the maximum excitation and emission wavelengths were 292 and 328 nm.[Results]8 kinds of vitamins were determined simultaneously for the first time, which were in good linear relationship within the detection range, with R2>0.999 0 and recovery rate exceeded 98%. There were 7 kinds of E vitamins in the flower of F. thunbergii Miq., among which D-α-tocotrienols was the highest that reached 181.606 mg/kg, however the contents of E vitamins in the pollens of F. thunbergii Miq. were relatively lower. [Conclusion] There are multiple kinds of E vitamins in the flowers and pollens of F. thunbergii Miq., comparatively speaking, E vitamins in the flower of F. thunbergii Miq. were more than that in pollen, which deserves further research. Besides, HPLC-FLD is convenient and efficiency that can be used for determining the contents of E vitamins.
Flowers of F. thunbergii Miq., Vitamin E, HPLC-FLD, China
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