KONG Feng-yun 1,2, ZHANG Qi-yao 1, GUAN Qun 1, JIAN Fu-qin 1, SUN Wei 1, WANG Yue 2 1. Department of Reproduction, The Second Affiliated Hospital of Shandong University of TCM, Jinan 250001, China; 2. Medical College of Shandong University JIAO Yue
Objective To explore the impacts of electro-acupuncture on embryo implanted potential and its molecular mechanism in patients with infertility of different syndromes. Methods Among infertile patients treated with in vitro fertilization and embryo transplantation (IVF-ET) combined with electro-acupuncture, 82 cases of kidney deficiency syndrome (group A), 74 cases of liver qi stagnation syndrome (group B) and 54 cases of phlegm dampness syndrome (group C) were selected. Patients in three groups all received a long program of ovarian hyperstimulation. Additionally, electro-acupuncture was applied before controlled ovarian hyperstimulation (COH) and in the process of ovarian hyperstimulation. Contents of human leukocyte antigen G (HLA-G) in the serum were determined in three groups respectively on the 2nd day of menstruation (M2) and the day of human Chorionic Gonadotropin (hCG) injection. Content of HLA-G in the culture solution of three groups was examined separately on the day of embryo transplantation. The fertilization rate, implantation rate and clinical pregnancy rate were observed for patients in three groups. Results Contents of HLA-G in the serum on the day of hCG injection and in the culture solution on the day of embryo transplantation in group A and B were significantly higher than those in group C (all P<0.05). However, there was no significant difference with contents of HLA-G in serum on M2 day among three groups. The high quality embryo rate in either group A (73.6%, 352/478) or group B (70.6%, 379/537) was significantly higher than that in group C (54.2%, 208/384) with significantly statistical difference (all P<0.01). But there were no significant differences in clinical pregnancy rate, fertilization rate and cleavage rate among three groups. Conclusion Electro-acupuncture combined could increase contents of HLA-G in the body and the level of HLA-G secreted from embryos of patients in the process of IVF-ET. Eventually, the pregnancy outcome and the pregnancy rate are improved. It showed better clinical effects of electro-acupuncture for patients with kidney deficiency and liver qi stagnation syndromes than those of phlegm dampness syndome.
infertility; female; electro-acupuncture; in vitro fertilization(IVF); embryo transplantation (ET); syndrome differentiatio
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