Surface Microstructure on Hydroxyapatite Spherules and Its Regulation on Stem Cells

【Author】

ZHI Wei;SHI Feng;LI Jing-Yu;ZHOU Teng;QU Shu-Xin;WANG Jian-Xin;ZHANG Cong;WENG Jie

【Institution】

Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University;The 309st Hospital of Chinese PLA;Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University

【Abstract】

HA spheres with different surface microstructures were prepared by a Sol-Gel route with various hydroxyapatite (HA)/chitin ratios. The surface morphologies, phase compositions, and biomimetic mineralization ability of samples were respectively analyzed with scanning electron microscopy (SEM), X-ray diffraction (XRD), and biomimetic mineralization test. Furthermore, the influences of the surface microstructure on biological behavior of bone marrow-derived mesenchymal stem cells were examined by Alamar blue, SEM, alkaline phosphatase assay and flow cytometry. When the mass ratio of HA/Chitin was increased from 4/1 to 35/1, the surface microstructure of HA spheres changed substantially, as shown by a reduction of micro-porosity from (35% ± 0.8%) to (10.4% ± 0.7%), decrease of surface roughness, and gradual disappearance of micro-creases. The result of biomimetic mineralization test showed that the surface microstructure had important influence on the ability of biomimetic mineralization for HA spheres. In vitro culture of bone marrow-derived mesenchymal stem cells demonstrated that the rougher sphere surface, with abundant micro-creases and micro-pores, supported cell spreading and proliferation. In comparison, the smoother sphere surface with fewer micro-pores effectively induced cell elongation and up-regulated ALP expression, which suggested stem cells osteogenic differentiation. Meanwhile, the surface microstructure of spherules also modulated the expression of characteristic antigen makers on the surface of stem cells.

【Keywords】

hydroxyapatite;surface microstructure;stem cell;biological behavior

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Total: 28 articles

  • [1] GUO Lai-Yang~1,ZHANG Jing-Wei~1,ZHAO Jing~1,WANG Jian-Xin~1,WENG Jie~1,ZHANG Cong~2 (1.Key Laboratory of Advanced Technologies of Materials,Ministry of Education,School of Materials Science and Engineering, Southwest Jiaotong University,Chengdu 610031,China;2.452nd Hospital of Chinese PLA,Chengdu 610021,China), Preparation and Characterization of Porous Scaffolds with Favourable Interpore Connectivity, Journal of Inorganic Materials,
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  • [4] Pamela Habibovic;;Huipin Yuan;;Chantal M. van der Valk;;Gert Meijer;;Clemens A. van Blitterswijk;;Klaas de Groot, 3D microenvironment as essential element for osteoinduction by biomaterials, Biomaterials,

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