ZHANG Jia-Min;WANG Tao;TANG Chun-Bo;WANG Qiao-Na;QIAN Hai-Mei;MIAO Run-Jie
College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics;College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics;Stomatological Hospital of Jiangsu Province;Stomatological Hospital of Jiangsu Province;College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics;College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics
A polydopamine/casein phosphopeptides (PDA/CPP) bilayer was prepared on the pure titanium surface with macro and micro pits formed by sandblasting with large-grit and acid-etching (SLA). The PDA interlayer with extraordinary adhesion and the capability of second functional modification, linked CPP, the natural biomolecules, to the SLA titanium surface via covalent bonds. This two-step immersion modification process was simple and feasible. Field emission scanning electronic microscope (FESEM), energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscppe (XPS) were utilized to analyze microtopography and elementary composition of PDA/CPP bilayer. Contact angle analysis, biomimetic mineralization examinations and human bone marrow stem cells (hBMSCs) experiments in vitro were conducted to verify the biocompatibility of the modified surfaces. The results showed that the PDA/CPP bilayer was successfully grafted onto the SLA substrates through the two-step immersion method. The bilayer decreased the contact angle of SLA with pure water from 47.7° to 25.5°, showing the improved hydrophilicity obviously. Compact hydroxyapatite (HA) layer was formed after SLA/PDA/CPP immersed in simulated body fluid (SBF) for 1 d, demonstrating that the bilayer accelerated the rate of biomineralization significantly. Besides, the PDA/CPP bilayer efficiently enhanced the cell attachment, cell proliferation and cell mineralization.
polydopamine;casein phosphopeptides;SLA;pure titanium;bio-activation
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