KGM/Gelatin/Nano HAP Scaffolds for Tissue Engineering of Intervertebral Disc Annulus Fibrosus


CHEN Xi-Liang;CHEN Qing-Hua;ZHUANG Ying;YAN Ting-Ting


Faculty of Materials Science and Engineering, Kunming University of Science and Technology;Faculty of Materials Science and Engineering, Kunming University of Science and Technology;Kunming General hospital of Chengdu Military Region;Faculty of Materials Science and Engineering, Kunming University of Science and Technology


Intervertebral disc degenerative disease has become a serious disease which affects people’s work and life, and the emergence of tissue engineering technology provides a new way to the treatment of intervertebral disc disease. In this research, Konjac glucomannan (KGM), gelatin, and nano hydroxyapatite (nano HAP) were used to prepare tissue engineering scaffolds for intervertebral disc annulus fibrosus by wet spinning and rolling film method. The composition, structures and morphologies of the scaffolds were analyzed by scanning electron microscope (SEM), X-ray diffractometer (XRD) and Fourier transform infrared (FT-IR) spectrometer. The compressive strength, water absorption, porosity, in vitro degradation, and cytotoxicity of scaffolds were also measured. The results show that the scaffolds are anisotropic structure, similar to natural annulus fibrosus. The scaffolds are porous, with their strength being improved by adding nano HAP. The strength of scaffolds prepared by wet spinning method is higher than that by rolling film method; the scaffolds cross-linked by glutaraldehyde are stronger than those by ammonia because of better nano HAP combination, and exhibit higher degradation rate. The water absorption and porosity of the scaffolds are over 700%, 66%–75%, respectively. This study provides a theoretical and experimental basis for further development of tissue engineering scaffolds for annulus fibrosus.


annulus fibrosus;tissue engineering scaffold;konjac glucomannan;gelatin;nano hydroxyapatite


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Total: 27 articles

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