Protein engineering of lignin degrading enzyme based on electron transfer pathway


Le Thanh Mai Pham;Yong Hwan Kim;


<正>The lignin peroxidase isozyme H8(LiPH8)from Phanerochaete chrysosporium has been demonstrated to directly oxidize nonphenolic lignin model compound throughout exposed Trp171 site.LiPH8 needs mediators for oxidation of some compounds such as polymeric lignin.The Trp171 surrounding amino acids have been suggested to play a key role for oxidation of veratryl alcohol(VA)or lignin derivatives in LiPH8.




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