Screening of gene-specific amplicons from metagenomes for improved biocatalysis

【Author】

Nobuya Itoh;Hiroshi Toda;

【Abstract】

<正>Metagenomics is an emerging tool for the isolation of novel genes.However,most previous approaches have featured metagenomic DNA extraction and subsequent Escherichia coli library construction,followed by sequence-or function/molecule-based screens of the new library.Such approaches are very time consuming.Although PCR amplification of truncated genes from metagenomes can facilitate the identification of genes,the available methods are inefficient in many cases,and often fail

【Keywords】

library;consuming;subsequent;Screening;inefficient;isolation;truncated;featured;facilitate;amplification;

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