Structure-based enzyme engineering combined with kinetic profiling of substrate libraries:alcohol dehydrogenases for efficient synthesis of bulky chiral alcohols

【Author】

Yao Nie;Yan Xu;Rong Xiao;Gaetano T.Montelione;Thomas Szyperski;

【Abstract】

<正>The alcohol dehydrogenase(ADH)-catalyzed reduction of prochiral ketones offers a valuable synthetic route given the chemo-,regio-,and stereoselectivity of this class of enzymes.However,most naturally occurring enzymes exhibit a rather narrow substrate profile.In contrast,ketones with two bulky groups are important precursors for anti-depressant,anti-asthmatic,cholesterol-lowering,and a-or(3-adrenergic drugs.Recently,we characterized the carbonyl reductase from Candida parapsilosis(CpRCR)

【Keywords】

alcohol;profiling;dehydrogenase;adrenergic;carbonyl;libraries;Candida;lowering;chiral;catalyzed;

References

To explore the background and basis of the node document

Springer Journals Database

Total: 18 articles

  • [1] M.M.Musa;R.S.Phillips, Catal.Sci.Technol,
  • [2] J.Kulig;R.C.Simon;C.A.Rose;S.M.Husain;M.Hackh;S.Ludeke;K.Zeitler;W.Kroutil;M.Pohl;D.Rother, Catal.Sci.Technol,
  • [3] A.Dennig;N.Lulsdorf;H.Liu;U.Schwaneberg, Angew.Chem.Int.Ed, Angewandte Chemie International Edition (Angewandte Chemie International Edition)
  • [4] S.Wang;Y.Xu;R.Zhang;B.Zhang;R.Xiao, Process Biochem, Process Biochemistry (Process Biochemistry)

More>>

Similar documents

Documents that have the similar content to the node document

Springer Journals Database

Total: 50 articles

More>>