Disorder prediction-based mutation of Bacillus naganoensis pullulanase for improvement of its activity and catalytic efficiency

【Author】

Xinye Wang;Yao Nie;Xiaoqing Mu;Yan Xu;Rong Xiao;

【Abstract】

<正>Pullulanase(EC 3.2.1.41)catalyzes hydrolysis of theα-1,6 glycosidic linkages in pullulan,amylopectin,and theα-andβ-limit dextrins of amylopectin1.During the saccharification process,glucoamylase and pullulanase act simultaneously on the substrate,and addition of pullulanase can improve the substrate conversion rate and reduce the amount of glucoamylase2.Thus,there is a great commercial

【Keywords】

pullulan;hydrolysis;mutation;truncation;cloned;residues;helpful;starch;displayed;Prediction;

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