High-level intra- and extra-cellular production of d-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis

【Author】

Jingqi Chen;Dawei Zhang;

【Abstract】

<正>D-Psicose 3-epimerase(DPEase)converts d-fructose into D-psicose which exists in nature in limited quantities and has key physiological functions.In this study,RDPE(DPEase from Ruminococcus sp.5_1_39BFAA)was successfully constitutively expressed in Bacillus subtilis,which is the first report of its kind.Three sugar-inducible promoters were compared,and the xylose-inducible promoter P xylA was

【Keywords】

fructose;physiological;quantities;promoter;deletion;sugar;inducer;fermentation;batch;secretion;

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