Discovery of a new bacterial lytic polysaccharide monooxygenase and its effects in oxidative degradation of chitin


Yong Zhao;Huiyan Zhang;Heng Yin;


Lytic polysaccharide monooxygenases(LPMOs)are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose.Considering the importance of LPMOs in biomass conversion and the large number of Ipmo genes in microorganisms,there are,however,only a few LPMOs have been well studied,especially for their effects in bioconversion of recalcitrant polysaccharide.In this study,a chitin-active AAIO family LPMO from Bacillus thuringiensis subsp.kurstaki,2J/LPMO10A,was discovered for the first time.This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin,however,interestingly,when colloidal chitin is used as the substrates,a ladder of oxidized oligosaccharides is observed.These results provide new insights into the action mode of LPMOs that may be affected by the substrates.




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