Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions

【Author】

Soo-Jin Yeom;Hyewon Lee;Yu Jung Kim;Seung-Goo Lee;

【Abstract】

<正>Introduction Plasmid-based gene expression is a fundamental tool in the field of biotechnology.However,overexpression of genes of interest with multi-copy plasmids often causes detrimental effects on host cells.To overcome this problem,chromosomal integration of target genes has been used for decades;however,insufficient protein expression occurred with this method.Here,we developed a novel cloning and

【Keywords】

antibiotics;tightly;chromosomal;cloning;biotechnology;overcome;Plasmid;plasmid;insufficient;expressing;

References

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Total: 2 articles

  • [1] Lee,J.et al, Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination, PLoS One,
  • [2] Lee,J.et al, Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination, PLoS One,

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