Cloning,purification and characterization of cold-adapted protease from Antarctic Enterobacter sp.PAMC 25617

【Author】

Pak Seojeong;Jong-il Choi;

【Abstract】

<正>In this study,Cold adapted protease gene from psychrophilic bacteria Enterobacter sp.PAMC 25617 was cloned,purified and was characterization.This protease was expression using E.coli expression system.This recombinant strain expressed the protease from Enterobacter sp.PAMC 25617 on inclusion body and expressed protease size is 67 kDa.The

【Keywords】

Enterobacter;protease;cloned;Cloning;purified;purification;adapted;inclusion;dialysis;affinity;

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