A novel in vitro system for gamete fusion in maize


Xiong Bo PENG, Meng Xiang SUN*, Hong Yuan YANG Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China


Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertilization mechanisms of plants. In this study, we developed a bovine serum albumin (BSA)-mediated fusion system for the in vitro fusion of maize gametes. The in vitro fusion of the isolated single egg cell and sperm cell of maize was observed microscopically in the BSA solution and the fertilized egg cell showed normal cell wall regeneration and nuclear division. The effects of the BSA concentration, pH value and calcium level on the efficiency of the maize gamete fusion were also assessed. BSA concentration and pH value did significantly affect the efficiency of the gamete fusion. Calcium was not necessary for the gamete fusion when BSA was present. The optimal solution for the gamete fusion contained 0.1% BSA, pH 6.0. The fusion frequency was as high as 96.7% in that optimal solution. This new in vitro fertilization system offers an alterna- tive tool for the in vitro study of fertilization mechanisms with much simpler manipulating procedure than PEG system, and it will be especially useful for the in vitro study of the calcium dynamics during plant fertilization.


in vitro fertilization, bovine serum albumin, calcium, maize.


To explore the background and basis of the node document

Springer Journals Database

Total: 4 articles

  • [1] In vitro fertilization of single, isolated gametes of maize mediated by electrofusion, Sexual Plant Reproduction,
  • [2] I. Dupuis;;C. Dumas, In vitro pollination as a model for studying fertilization in maize ( Zea mays L.), Sexual Plant Reproduction,
  • [3] M. -X. Sun;;A. Moscatelli;;H. -Y. Yang;;M. Cresti, In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy, Sexual Plant Reproduction,
  • [4] H. Q. Tian;;S. D. Russell, Micromanipulation of male and female gametes of Nicotiana tabacum : II. Preliminary attempts for in vitro fertilization and egg cell culture, Plant Cell Reports,