A Complete Embryonic Developmental Table of Microhyla fissipes(Amphibia, Anura, Microhylidae)

【Author】

Shouhong WANG;Lanying ZHAO;Lusha LIU;Dengwei YANG;Janak Raj KHATIWADA;Bin WANG;Jianping JIANG;Chengdu Institute of Biology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;

【Institution】

Chengdu Institute of Biology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;

【Abstract】

Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches(range 209–564 eggs) of ornamented pygmy frog Microhyla fissipes(Amphibia, Anura, Microhylidae) were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated: 1) early embryonic development period(stages 1–28), from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature(WT) 23–25℃; 2) larval development period(stages 29–45), from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5℃, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease.

【Keywords】

Microhylidae;;embryonic stage;;external characters;;morphogenesis

References

To explore the background and basis of the node document

Springer Journals Database

Total: 28 articles

  • [1] XIONG Rong-Chuan1,2, JIANG Jian-Ping1, FEI Liang1, WANG Bin1,2, YE Chang-Yuan1 (1. Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu 610041, China; 2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China), Embryonic development of the concave-eared torrent frog with its significance on taxonomy, Zoological Research,
  • [2] XU Meng-yang1,XU Jian2(1.College Life Science,Tianjin Normal University,Tianjin 300387,China;2.College of Life Biology,Shaoguan University,Shaoguan,Guangdong Province 512005,China), Preliminary Observations on Post-embryonic Development of Microhyla ornata in North Guangdong, Sichuan Journal of Zoology,
  • [3] Lu-Sha LIU;Lan-Ying ZHAO;Shou-Hong WANG;Jian-Ping JIANG;Chengdu Institute of Biology, Chinese Academy of Sciences;University of Chinese Academy of Sciences;, Research proceedings on amphibian model organisms, Zoological Research,
  • [4] GENG Baorong;WEN Qing;ZHANG Qiujin(College of Bioengineering, Fujian Normal University Fuzhou 350007), EARLY EMBRYONIC DEVELOPMENT IN MICROHYLA ORNATA, CHINESE JOURNAL OF ZOOLOGY,

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