Cysteinyl leukotriene receptors CysLT_1 and CysLT_2 are upregulated in acute neuronal injury after focal cerebral ischemia in mice


Yan-jun ZHANG~(2,3)Lei ZHANG~2 Yi-lu YE~2 San-hua FANG~2 Yu ZHOU~2 Wei-ping ZHANG~2 Yun-bi LU~2 Er-qing WEI~(2,4)2 Department of Pharmacology,School of Medicine,Zhejiang University,Hangzhou 310058;3 Zhejiang Provincial Centre for Disease Control and Prevention,Hangzhou 310009,China


Aim:To determine whether cysteinyl leukotriene receptors(CysLT_1 and CysLT_2) are upregulated in acute neuronal injury after focal cerebral ischemia in mice,and to confirm CysLT_1 receptor localization.Methods:After permanent focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO),neurological deficits and neuron loss were determined at various time points within 48h.The mRNA expressions of CysLT_1 and CysLT_2 receptors in the brain were analyzed by RT-PCR.CysLT_1 receptor localization was detected by double immunofluorescence. Results:Neurological deficits and neuron loss were found 6,24 and 48h after MCAO.The mRNA expressions of both CysLT_1 and CysLT_2 receptors were upregulated in the ischemic hemisphere 1,24,and 48 h after MCAO with peaks at 24 h.The CysLT_1 receptor was selectively localized in neurons 24 h after MCAO. Conclusion:CysLT_1 and CysLT_2 receptors are upregulated in acute neuronal in- jury after focal cerebral ischemia,and the CysLT_1 receptor is localized in neurons after ischemia.


cysteinyl leukotriene receptor;;cerebral ischemia;;neuronal injury;;mRNA expression


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  • [1] A. Muralikrishna Rao;;J. F. Hatcher;;M. S. Kindy;;R. J. Dempsey, Arachidonic Acid and Leukotriene C 4 : Role in Transient Cerebral Ischemia of Gerbils, Neurochemical Research,

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