Effects of different LEDs light spectrum on the growth,leaf anatomy,and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse

【Author】

CHEN Li-li;ZHANG Kai;GONG Xiao-chen;WANG Hao-ying;GAO You-hui;WANG Xi-quan;ZENG Zhao-hai;HU Yue-gao;College of Agronomy and Biotechnology,China Agricultural University;Key Laboratory of Crop Water Use and Regulation,Ministry of Agriculture/Farmland Irrigation Research Institute,Chinese Academy of Agricultural Sciences;

【Institution】

College of Agronomy and Biotechnology,China Agricultural University;Key Laboratory of Crop Water Use and Regulation,Ministry of Agriculture/Farmland Irrigation Research Institute,Chinese Academy of Agricultural Sciences;

【Abstract】

Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m~(-2)s~(-1) total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable; BB and RB could be used as alternatives.

【Keywords】

potato plantlets in vitro;;LEDs light spectrum;;leaf anatomy;;chloroplast ultrastructure;;minituber production

References

To explore the background and basis of the node document

Springer Journals Database

Total: 24 articles

  • [1] Lili CHEN;Xuzhang XUE;Yadong YANG;Fei CHEN;Jie ZHAO;Xiquan WANG;Alam Tariful KHAN;Yuegao HU;College of Agronomy and Biotechnology, China Agricultural University;Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences;, Effects of red and blue LEDs on in vitro growth and microtuberization of potato single-node cuttings, Frontiers of Agricultural Science and Engineering,
  • [2] Nathalie Korbee;;Félix L. Figueroa;;José Aguilera, Effect of light quality on the accumulation of photosynthetic pigments, proteins and mycosporine-like amino acids in the red alga Porphyra leucosticta (Bangiales, Rhodophyta), Journal of Photochemistry & Photobiology, B: Biology,
  • [3] Andrea Furtado Macedo;;Marcos Vinícius Leal-Costa;;Eliana Schwartz Tavares;;Celso Luiz Salgueiro Lage;;Maria Apparecida Esquibel, The effect of light quality on leaf production and development of in vitro -cultured plants of Alternanthera brasiliana Kuntze, Environmental and Experimental Botany,
  • [4] ANDREW C. SCHUERGER;;CHRISTOPHER S. BROWN;;ELIZABETH C. STRYJEWSKI, Anatomical Features of Pepper Plants ( Capsicum annuum L.) Grown under Red Light-emitting Diodes Supplemented with Blue or Far-red Light, Annals of Botany,

More>>

Similar documents

Documents that have the similar content to the node document

Springer Journals Database

Total: 20 articles

  • [1] HCN evolution and changes of activity of P-cyanoalanine synthase in potato tubers, Chinese Science Bulletin, 1997 (12)
  • [2] Preliminary Studies on the Production of Artificial Seeds by Encapsutating Microtubers of Potato, The Journal of Northeast Agricultural University(English Edition), 1996 (02)
  • [3] Genetic Diversity Analysis of PVY Resistant Potato Varieties (Clones) by RAPD Markers, Journal of Northeast Agricultural University(English Edition), 2006 (01)
  • [4] Xiaoding GUO;Yizhi XIE;Zhaodong JIA;Peiyong MA;Xiaofeng BIAN;, Study on Sweet Potato Stem Nematode Disease, Plant Diseases and Pests, 2012 (02)

More>>