MicroRNA-22 inhibits proliferation and promotes differentiation of satellite cells in porcine skeletal muscle

【Author】

Hong Quyen DANG;XU Gu-li;HOU Lian-jie;XU Jian;HONG Guang-liang;Chingyuan HU;WANG Chong;National Engineering Research Center for Breeding Swine Industry/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/College of Animal Science,South China Agricultural University;Bac Giang Agricultural and Forestry University;Department of Human Nutrition,Food and Animal Sciences,College of Tropical Agriculture and Human Resources,University of Hawaii at Manoa;

【Institution】

National Engineering Research Center for Breeding Swine Industry/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/College of Animal Science,South China Agricultural University;Bac Giang Agricultural and Forestry University;Department of Human Nutrition,Food and Animal Sciences,College of Tropical Agriculture and Human Resources,University of Hawaii at Manoa;

【Abstract】

Pig is an important economic animal in China. Improving meat quality and meat productivity is a long time issue in animal genetic breeding. Micro RNAs(mi RNAs) are short non-coding RNAs that participate in various biological processes, such as muscle development and embryogenesis. mi R-22 differentially expresses in embryonic and adult skeletal muscle. However, the underlying mechanism is unclear. In this study, we investigated mi R-22 function in proliferation and differentiation of porcine satellite cells(PSCs) in skeletal muscle. Our data show that mi R-22 expressed in both proliferation and differentiated PSCs and is significantly upregulated(P<0.05) during differentiation. After treated with the mi R-22 inhibitor, PSCs proliferation was significantly increased(P<0.05), as indicated by the up-regulation(P<0.01) of cyclin D1(CCND1), cyclin B1(CCNB1) and down-regulation(P<0.05) of P21. Conversely, over-expression of mi R-22 resulted in opposite results. Differentiation of PSCs was significantly suppressed(P<0.05), evidenced by two major myogenic markers: myogenin(Myo G) and myosin heavy chain(My HC), after transfecting the PSCs with mi R-22 inhibitor. Opposite results were demonstrated in the other way around by transfection with mi R-22 mimics. In conclusion, the data from this study indicated that mi R-22 inhibited the PSCs proliferation but promoted their differentiation.

【Keywords】

miR-22;;skeletal muscle;;porcine satellite cells;;proliferation;;differentiation

References

To explore the background and basis of the node document

Springer Journals Database

Total: 20 articles

  • [1] Hristo B Houbaviy;;Michael F Murray;;Phillip A Sharp, Embryonic Stem Cell-Specific MicroRNAs, Developmental Cell,
  • [2] W.H. Davin Townley-Tilson;;Thomas E. Callis;;DaZhi Wang, MicroRNAs 1, 133, and 206: Critical factors of skeletal and cardiac muscle development, function, and disease, International Journal of Biochemistry and Cell Biology,
  • [3] Andrew H Williams;;Ning Liu;;Eva van Rooij;;Eric N Olson, MicroRNA control of muscle development and disease, Current Opinion in Cell Biology,
  • [4] David P. Bartel, MicroRNAs: Target Recognition and Regulatory Functions, Cell,

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