Huang Xiaoyong, Yin Zhengqin, WangShijun, Zeng Yuxiao Southwest Eye Hospital, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
<正> Objective To isolate the retinal stem cells from optic cup of embryonic rats during tail bud stage and identify their properties.Methods The cells, which are able to form single-cell cloning, were isolated from the optic cup of embryonic rats during tail bud stage and cultured with serum-free media. Immunofluorescence technique was employed to detect BrdU and CHX10 antigen in the retinal stem cells and specific antigen of mature retinal cells after differentiation.
retinal stem cells; embryonic rat
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