Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat


Eva H.C.Tang;George P.M.Leung;Amy C.Y.Lo;Sookja K.Chung;Ricky Y.K.Man;Paul M.Vanhoutte;


<正>Expression pattern of prostanoid synthases on freshly isolated endpthelial cells and its respective receptors on smooth muscle cells of the thoracic aorta of eight-month old Wistar Kyoto rat (WKY) and spontaneously hypertensive rat (SHR) are assessed. Total RNA was isolated, converted into cDNA and used for quantitative real-time polymerase chain reaction (PCR) using SYBR green fluorescent dye to quantify the accumulation of double-stranded PCR




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