Removal of phenol using horseradish peroxidase immobilized in the nano-scale porous structural material


Hyo Jun Kim;Han S.Kim;


In this study horseradish peroxidase(HRP) was immobilized in the nano-scale porous structural material for the purpose of protecting enzyme and its activity.Kinetic rate of the immobilized enzyme for the removal of phenolic compounds were evaluate to examine its functional activity.Montmorillonite(2:1 clay mineral) was employed as an inorganic backbone frame material and humic acid was coated on the surface of montmorillonite,mediating cross-linking of enzymes with the inorganic clay mineral.The immobilizing yield was fairly high ranging 16 to 22.2%(w/w) and the immobilization was achieved effectively in a reasonably short period(-0.5hr).Activity of the HRP immobilized in the inorganic clay mineral was well preserved,confirming that the immobilized HRP can catalyze the oxidative cross-coupling to remove phenolic compounds.The parameters of Michaelis-Menten model for both the original naked and the immobilized HRPs were not markedly different,supporting that the HRP immobilized can catalyze the polymerization of phenolic compounds almost at the same rate of the original enzyme.Further studies for clearer understanding of the underneath mechanisms are required,but the results presented in this study are,to the best of our knowledge,the first attempt to evaluate the activity of immobilized enzyme for environmental purposes and they are useful to develop a new biological treatment technique for hazardous substances in the green and ubiquitous eco-city in the future.


phenol;HRP;immobilization;montmorillonite;humic acid


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Total: 24 articles

  • [1] Yi-Chen Lai;;Sung-Chyr Lin, Application of immobilized horseradish peroxidase for the removal of p -chlorophenol from aqueous solution, Process Biochemistry,
  • [2] K. Nazari;;N. Esmaeili;;A. Mahmoudi;;H. Rahimi;;A.A. Moosavi-Movahedi, Peroxidative phenol removal from aqueous solutions using activated peroxidase biocatalyst, Enzyme and Microbial Technology,
  • [3] J.L. Gómez;;A. Bódalo;;E. Gómez;;J. Bastida;;A.M. Hidalgo;;M. Gómez, Immobilization of peroxidases on glass beads: An improved alternative for phenol removal, Enzyme and Microbial Technology,
  • [4] Monika Wagner;;James A Nicell, Detoxification of phenolic solutions with horseradish peroxidase and hydrogen peroxide, Water Research,


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