A new fluorimetric assay for spermine by recombinant spermine oxidase coupled HRP reaction

【Author】

Peng fan;Xiao fangxiang;Han li;Wang yanlin;

【Abstract】

<正>A rapid and sensitive tluorimetric method for determination of spermine in tumor cell was described.The method was performed by purified,recombinant PAOh1/SMO protein coupled with horseradish peroxidase reaction,in which non-fluorogenic substrate HPPA can liberate fluorescence after accepting electron.The Ex was 340nm and the Em was 430nm,respectively.The

【Keywords】

HRP;A new fluorimetric assay for spermine by recombinant spermine oxidase coupled HRP reaction;

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