Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions

Title browse > Conferences > The 14th China-Japan-Korea Joint Symposium on Enzy... > Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions

Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions

【Author】

Soo-Jin Yeom;Hyewon Lee;Yu Jung Kim;Seung-Goo Lee;

【Abstract】

<正>Introduction Plasmid-based gene expression is a fundamental tool in the field of biotechnology.However,overexpression of genes of interest with multi-copy plasmids often causes detrimental effects on host cells.To overcome this problem,chromosomal integration of target genes has been used for decades;however,insufficient protein expression occurred with this method.Here,we developed a novel cloning and

【Keywords】

RMT;Long-term stable and tightly controlled expression of recombinant proteins in antibiotics-free conditions;

The 14th China-Japan-Korea Joint Symposium on Enzyme Engineering Conference Proceeding

References

To explore the background and basis of the node document

Total: 2 articles

[1] Lee,J.et al, Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination, PLoS One,
[2] Lee,J.et al, Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination, PLoS One,

Home

Collections By Topic

Title Browse