K.Y.PU,R.Y.ZHAN,J.B.SHI,and B.LIU~* Department of Chemical and Biomolecular Engineering National University of Singapore Singapore 117576
<正>Molecular recognition and quantitative determination of heparin are vital steps in chemical sensing of polyanionic carbohydrates.Traditional clinical procedures for heparin sensing are indirect,which rely on the measurement of activated clotting time(ACT),or activated partial thromboplastin time(aPTT).These methods are not sufficiently reliable,accurate,and amenable to clinical settings.In this respect,we have successfully developed three types of conjugated polyelectrolyte(CPE) based heparin optical sensing strategies with different signal outputs.The different signal outputs originate from the ingenious design of polymer's chemical structure,which subsequently provide these heparin sensors with individual advantages to satisfy the different requirements for specific applications.The work mechanism for these biosensors similarly relies on the strong electrostatic attractions between heparin and CPE in combination with the environment-sensitive optical properties of CPE. For CPE-based multicolor heparin sensing,a polymer is designed to show fluorescent color variation according to the heparin concentration.With increasing heparin concentration,the fluorescent color of the polymer solution gradually changes from blue to orange,which allows heparin quantification in the range of 30 nM to 48μM.For CPE-based light-up heparin sensor,a CPE with charge-transfer character was designed to show very weak intrinsic fluorescence in aqueous solution.Upon addition of heparin into the polymer solution,the polymer fluorescence increases progressively as a function of the heparin concentration,leading to a light-up sensing of heparin with a quantification range of 30 nM to 72μM.For CPE-based colorimetric heparin sensor,a cationic water-soluble polythiophene was used,which showed large absorption variation upon conformational changes.The polymer solution was yellow in the absence of heparin,while it gradually turned to red with increased heparin concentrations.
Heparin;;multi-color sensing;;fluorescence turn-on sensing;;colorimetric sensing
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